Authors: Shuming Dong, Yushan Dong, Bin Liu, Jing Liu, Shikai Liu, Zhiyu Zhao, Wenting Li, Boshi Tian, Ruoxi Zhao, Fei He, Shili Gai, Ying Xie, Piaoping Yang, Yanli Zhao
Published: 2021-12-05
Source: Full article
AbstractClinical applications of nanozyme‐initiated chemodynamic therapy (NCDT) have been severely limited by the poor catalytic efficiency of nanozymes, insufficient endogenous hydrogen peroxide (H2O2) content, and its off‐target consumption. Herein, the authors developed a hollow mesoporous Mn/Zr‐co‐doped CeO2 tandem nanozyme (PHMZCO‐AT) with regulated multi‐enzymatic activities, that is, the enhancement of superoxide dismutase (SOD)‐like and peroxidase (POD)‐like activities and inhibition of catalase (CAT)‐like activity. PHMZCO‐AT as a H2O2 homeostasis disruptor promotes H2O2 evolution and restrains off‐target elimination of H2O2 to achieve intensive NCDT. PHMZCO‐AT with SOD‐like activity catalyzes endogenous superoxide anion (O2•−) into H2O2 in the tumor region. The suppression of CAT activity and depletion of glutathione by PHMZCO‐AT largely weaken the off‐target decomposition of H2O2 to H2O. Elevated H2O2 is then catalyzed by the downstream POD‐like activity of PHMZCO‐AT to generate toxic hydroxyl radicals, further inducing tumor apoptosis and death. T1‐weighted magnetic resonance imaging and X‐ray computed tomography imaging are also achieved using PHMZCO‐AT due to the existence of paramagnetic Mn2+ and the high X‐ray attenuation ability of elemental Zr, permitting in vivo tracking of the therapeutic process. This work presents a typical paradigm to achieve intensive NCDT efficacy by regulating multi‐enzymatic activities of nanozymes to perturb the H2O2 homeostasis.