Authors: Tao Yang, Rong Liu, Yingfeng Luo, Songnian Hu, Dong Wang, Chenyu Wang, Manish K. Pandey, Song Ge, Quanle Xu, Nana Li, Guan Li, Yuning Huang, Rachit K. Saxena, Yishan Ji, Mengwei Li, Xin Yan, Yuhua He, Yujiao Liu, Xuejun Wang, Chao Xiang, Rajeev K. Varshney, Hanfeng Ding, Shenghan Gao, Xuxiao Zong
Published: 2022-09-22
DOI: 10.1038/s41588-022-01172-2
Source: Full article
AbstractComplete and accurate reference genomes and annotations provide fundamental resources for functional genomics and crop breeding. Here we report a de novo assembly and annotation of a pea cultivar ZW6 with contig N50 of 8.98 Mb, which features a 243-fold increase in contig length and evident improvements in the continuity and quality of sequence in complex repeat regions compared with the existing one. Genome diversity of 118 cultivated and wild pea demonstrated thatPisum abyssinicumis a separate species different fromP. fulvumandP. sativumwithinPisum. Quantitative trait locus analyses uncovered two known Mendel’s genes related to stem length (Le/le) and seed shape (R/r) as well as some candidate genes for pod form studied by Mendel. A pan-genome of 116 pea accessions was constructed, and pan-genes preferred inP. abyssinicumandP. fulvumshowed distinct functional enrichment, indicating the potential value of them as pea breeding resources in the future.