Authors: Jiaming Qian, Yu Cao, Ying Bi, Hongjun Wu, Yongtao Liu, Qian Chen, Chao Zuo
Published: 2023-02-07
DOI: 10.1186/s43593-022-00035-x
Source: Full article
AbstractStructured illumination microscopy (SIM) is one of the powerful super-resolution modalities in bioscience with the advantages of full-field imaging and high photon efficiency. However, artifact-free super-resolution image reconstruction requires precise knowledge about the illumination parameters. The sample- and environment-dependent on-the-fly experimental parameters need to be retrieved a posteriori from the acquired data, posing a major challenge for real-time, long-term live-cell imaging, where low photobleaching, phototoxicity, and light dose are a must. In this work, we present an efficient and robust SIM algorithm based on principal component analysis (PCA-SIM). PCA-SIM is based on the observation that the ideal phasor matrix of a SIM pattern is of rank one, leading to the low complexity, precise identification of noninteger pixel wave vector and pattern phase while rejecting components that are unrelated to the parameter estimation. We demonstrate that PCA-SIM achieves non-iteratively fast, accurate (below 0.01-pixel wave vector and 0.1$$\%$$