Authors: Yanjiao Li, Yunhao Wang, Maria Vera-Rodriguez, Leif Christopher Lindeman, Linda Ellevog Skuggen, Erik M. K. Rasmussen, Ingunn Jermstad, Shaista Khan, Madeleine Fosslie, Trine Skuland, Marie Indahl, Sherif Khodeer, Eva Kristine Klemsdal, Kang-Xuan Jin, Knut Tomas Dalen, Peter Fedorcsak, Gareth D. Greggains, Mads Lerdrup, Arne Klungland, Kin Fai Au, John Arne Dahl
Published: 2023-06-22
DOI: 10.1038/s41587-023-01831-7
Source: Full article
AbstractCurrent N6-methyladenosine (m6A) mapping methods need large amounts of RNA or are limited to cultured cells. Through optimized sample recovery and signal-to-noise ratio, we developed picogram-scale m6A RNA immunoprecipitation and sequencing (picoMeRIP–seq) for studying m6A in vivo in single cells and scarce cell types using standard laboratory equipment. We benchmark m6A mapping on titrations of poly(A) RNA and embryonic stem cells and in single zebrafish zygotes, mouse oocytes and embryos.