Apoplastic barriers are essential for nodule formation and nitrogen fixation in

Authors: Defeng Shen, Nikola Micic, Rafael E. Venado, Nanna Bjarnholt, Christoph Crocoll, Daniel Pergament Persson, Sebastian Samwald, Stanislav Kopriva, Philip Westhoff, Sabine Metzger, Ulla Neumann, Ryohei Thomas Nakano, Macarena MarĂ­n Arancibia, Tonni Grube Andersen

Published: 2025-03-20

DOI: 10.1126/science.ado8680

Source: Full article


Abstract

Establishment of the apoplastic root barrier known as the Casparian strip occurs early in root development. In legumes, this area overlaps with nitrogen-fixing nodule formation, which raises the possibility that nodulation and barrier formation are connected. Nodules also contain Casparian strips, yet, in this case, their role is unknown. We established mutants with defective barriers in