Abstract 333: Cardiomyocyte Hypercontractility is an Adaptational Response to Stiffness in High-Fat Diet Mice

Authors: Johannes V Janssens, Antonia J Raaijmakers, Parisa Koutsifeli, Kimberley M Mellor, Claire L Curl, Lea M Delbridge

Published: 2019-10-16

DOI: 10.1161/res.125.suppl_1.333

Source: Full article

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Abstract

Diabetic heart disease typically features a restrictive/ hypertrophic cardiac phenotype characterised by diastolic dysfunction with preserved systolic performance. Little is known about the early adaptational mechanisms that underpin maintenance of systolic performance in diabetic diastolic dysfunction and how this may contribute to disease progression. This study aimed to evaluate cardiomyocyte contractility in a high-fat diet (HFD) mouse model of diastolic dysfunction. Echocardiography (GE Vivid 9) was performed in 33 wk old male C57Bl/6 mice fed a high-fat diet (HFD, 43% kcal fat, 24 wks duration). Isolated cardiomyocytes (paced 2Hz, 2.0mM Ca