Authors: Jining Yang, Xiaoying Zhang, Shen Ao, Xuewei Xia, Guo Yu, Xiaoyan Wu, Bing Xu, Miyuraj Withanage, Min Zhou, Jie Liu, Wei E Wang, Erliang Zeng, Ying Tian, Weizhong Zhu, Steven R Houser, Yang K Xiang, Chunyu Zeng, Mantian Mi, Xiongwen Chen
Published: 2019-10-16
DOI: 10.1161/res.125.suppl_1.360
Source: Full article
In adult mammalian hearts, the ventricles are composed of mostly binucleated myocytes (BVMs) and some mononucleated myocytes (MVMs), which have different properties. However, if the gene expression profile and pathological responses of these two populations of myocytes are different have not reported. We found that these two populations of myocytes differentially expressed 250 genes. While many RNA synthesis and processing genes, and some antiapoptotic genes were highly expressed in MVMs, BVMs had genes related to self-destruction (autophagy and ubiquitination system genes) enriched. In general, expression of genes involved in carbonhydrate metabolism and cell proliferation was also more but the expression of genes involved in fatty acid metabolism was less in MVMs. The expression of some of these genes was confirmed in single-molecule pulldown (SiMPull) method in separated MVMs and BVMs. MVMs grew more in pressure overloaded hearts. MVMs were more resistant to apoptosis induced by β-adrenergic stimulation both