Authors: Ligang Fan, Tingting Wang, Canfeng Hua, Wenju Sun, Xiaoyu Li, Lucas Grunwald, Jingui Liu, Nan Wu, Xiaolong Shao, Yimeng Yin, Jian Yan, Xin Deng
Published: 2020-10-02
DOI: 10.1038/s41467-020-18744-7
Source: Full article
AbstractPseudomonas syringae is a Gram-negative and model pathogenic bacterium that causes plant diseases worldwide. Here, we set out to identify binding motifs for all 301 annotated transcription factors (TFs) of P. syringae using HT-SELEX. We successfully identify binding motifs for 100 TFs. We map functional interactions between the TFs and their targets in virulence-associated pathways, and validate many of these interactions and functions using additional methods such as ChIP-seq, electrophoretic mobility shift assay (EMSA), RT-qPCR, and reporter assays. Our work identifies 25 virulence-associated master regulators, 14 of which had not been characterized as TFs before.