Authors: Bin Shao, Jayan Rammohan, Daniel A. Anderson, Nina Alperovich, David Ross, Christopher A. Voigt
Published: 2021-03-05
DOI: 10.1038/s41467-021-21734-y
Source: Full article
AbstractAccurate measurements of promoter activities are crucial for predictably building genetic systems. Here we report a method to simultaneously count plasmid DNA, RNA transcripts, and protein expression in single living bacteria. From these data, the activity of a promoter in units of RNAP/s can be inferred. This work facilitates the reporting of promoters in absolute units, the variability in their activity across a population, and their quantitative toll on cellular resources, all of which provide critical insights for cellular engineering.