Shedding light on the base-pair opening dynamics of nucleic acids in living human cells

Authors: Yudai Yamaoki, Takashi Nagata, Keiko Kondo, Tomoki Sakamoto, Shohei Takami, Masato Katahira

Published: 2022-11-29

DOI: 10.1038/s41467-022-34822-4

Source: Full article

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Abstract

AbstractBase-pair opening is a fundamental property of nucleic acids that plays important roles in biological functions. However, studying the base-pair opening dynamics inside living cells has remained challenging. Here, to determine the base-pair opening kinetics inside living human cells, the exchange rate constant ($${k}_{{{{{{\rm{ex}}}}}}}$$