Authors: Mai An Le, Jennifer Dang, Cameron Stinson, Devani Johnson, Logan Clair, James Ramsarran, Desmond Boakye Tanoh, Annam Humayun, Juliet Melnik, Tameka Dean, Qian CHEN, Robert Barsotti, Lindon Young
Published: 2024-10-09
DOI: 10.1161/res.135.suppl_1.we092
Source: Full article
Introduction: Naltrindole (NTI), known as a delta opioid receptor antagonist, inhibited phorbol 12-myristate 13-acetate (PMA) induced superoxide (SO) release in polymorphonuclear leukocytes (PMNs). PMNs do not express opioid receptors. We propose NTI reduces PMN SO by reducing intracellular calcium (Ca2+). To test this hypothesis, we did experiments using KB-R7943 (KB) as a positive control, known to reduce intracellular Ca2+ via inhibition of the reverse mode Na+/Ca2+ exchanger. Naloxone (NX), a broad-spectrum opioid receptor antagonist, was used as a negative control. We predict that NTI will diminish PMA-induced PMN SO release similar to KB, while NX will have no effect, and that there will be no difference in cell viability compared to vehicle controls.