Inhibition of CRISPR-Cas12a DNA targeting by nucleosomes and chromatin

Authors: Isabel Strohkendl, Fatema A. Saifuddin, Bryan A. Gibson, Michael K. Rosen, Rick Russell, Ilya J. Finkelstein

Published: 2021-03-10

DOI: 10.1126/sciadv.abd6030

Source: Full article

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Abstract

CRISPR-Cas12a cuts DNA in liquid-like chromatin condensates but is inhibited near nucleosomes.