Light-induced protein structural dynamics in bacteriophytochrome revealed by time-resolved x-ray solution scattering

Authors: Sang Jin Lee, Tae Wu Kim, Jong Goo Kim, Cheolhee Yang, So Ri Yun, Changin Kim, Zhong Ren, Indika Kumarapperuma, Jane Kuk, Keith Moffat, Xiaojing Yang, Hyotcherl Ihee

Published: 2022-05-27

DOI: 10.1126/sciadv.abm6278

Source: Full article

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Abstract

Bacteriophytochromes (BphPs) are photoreceptors that regulate a wide range of biological mechanisms via red light–absorbing (Pr)–to–far-red light–absorbing (Pfr) reversible photoconversion. The structural dynamics underlying Pfr-to-Pr photoconversion in a liquid solution phase are not well understood. We used time-resolved x-ray solution scattering (TRXSS) to capture light-induced structural transitions in the bathy BphP photosensory module of