Authors: Samuel Confederat, Seungheon Lee, Der Vang, Dimitrios Soulias, Fabio Marcuccio, Timotheus I. Peace, Martin Andrew Edwards, Pietro Strobbia, Devleena Samanta, Christoph Wälti, Paolo Actis
Published: 2023-08-31
Source: Full article
AbstractNanopore sensing has been successfully used to characterize biological molecules with single‐molecule resolution based on the resistive pulse sensing approach. However, its use in nanoparticle characterization has been constrained by the need to tailor the nanopore aperture size to the size of the analyte, precluding the analysis of heterogeneous samples. Additionally, nanopore sensors often require the use of high salt concentrations to improve the signal‐to‐noise ratio, which further limits their ability to study a wide range of nanoparticles that are unstable at high ionic strength. Here, a new paradigm in nanopore research that takes advantage of a polymer electrolyte system to comprise a conductive pulse sensing approach is presented. A finite element model is developed to explain the conductive pulse signals observed and compare these results with experiments. This system enables the analytical characterization of heterogeneous nanoparticle mixtures at low ionic strength