Authors: Sierra N. Cullati, Kazutoshi Akizuki, Jun-Song Chen, Jared L. Johnson, Tomer M. Yaron-Barir, Lewis C. Cantley, Kathleen L. Gould
Published: 2024-05-10
Source: Full article
CK1 kinases participate in many signaling pathways, and their regulation is of meaningful biological consequence. CK1s autophosphorylate their C-terminal noncatalytic tails, and eliminating these tails increases substrate phosphorylation in vitro, suggesting that the autophosphorylated C-termini act as inhibitory pseudosubstrates. To test this prediction, we comprehensively identified the autophosphorylation sites on