Cryo-shocked tumor cells deliver CRISPR-Cas9 for lung cancer regression by synthetic lethality

Authors: Feng Liu, Minhang Xin, Huiheng Feng, Wentao Zhang, Ziyan Liao, Tao Sheng, Ping Wen, Qing Wu, Tingxizi Liang, Jiaqi Shi, Ruyi Zhou, Kaixin He, Zhen Gu, Hongjun Li

Published: 2024-03-29

DOI: 10.1126/sciadv.adk8264

Source: Full article


Abstract

Although CRISPR-mediated genome editing holds promise for cancer therapy, inadequate tumor targeting and potential off-target side effects hamper its outcomes. In this study, we present a strategy using cryo-shocked lung tumor cells as a CRISPR-Cas9 delivery system for cyclin-dependent kinase 4 (