Authors: Aviva Geretz, Philip K. Ehrenberg, Robert J. Clifford, Alexandre Laliberté, Caterina Prelli Bozzo, Daina Eiser, Gautam Kundu, Lauren K. Yum, Richard Apps, Matthew Creegan, Mohamed Gunady, Shida Shangguan, Eric Sanders-Buell, Carlo Sacdalan, Nittaya Phanuphak, Sodsai Tovanabutra, Ronnie M. Russell, Frederic Bibollet-Ruche, Merlin L. Robb, Nelson L. Michael, Julie A. Ake, Sandhya Vasan, Denise C. Hsu, Beatrice H. Hahn, Frank Kirchhoff, Rasmi Thomas
Published: 2023-08-02
DOI: 10.1126/scitranslmed.adg0873
Source: Full article
Host restriction factors play key roles in innate antiviral defense, but it remains poorly understood which of them restricts HIV-1 in vivo. Here, we used single-cell transcriptomic analysis to identify host factors associated with HIV-1 control during acute infection by correlating host gene expression with viral RNA abundance within individual cells. Wide sequencing of cells from one participant with the highest plasma viral load revealed that intracellular viral RNA transcription correlates inversely with expression of the gene