Authors: Francesco Carraro, Margherita Aghito, Simone Dal Zilio, Heimo Wolinski, Christian J. Doonan, Bernd Nidetzky, Paolo Falcaro
Published: 2024-11-24
Source: Full article
AbstractThe one‐pot synthesis of multicomponent hydrogen‐bonded organic framework (HOF) biocomposites is reported. The co‐immoblization of enzymes and magnetic nanoparticles (MNPs) into the HOF crystals yielded biocatalysts (MNPs‐enzyme@BioHOF‐1) with dynamic localization properties. Using a permanent magnet, it is possible to separate the MNPs‐enzyme@BioHOF‐1 particles from a solution. Catalase (CAT) and glucose oxidase (GOx) show increased retention of their activity when coimmobilized with MNPs. MNPs‐GOx@BioHOF‐1 biocomposites are used to prepare a proof‐of‐concept glucose microfluidic biosensor, where a magnet allow to position and keep in place the biocomposite inside a microfluidic chip. The magnetic response of these biocatalysts can pave the way for new applications for the emerging HOF biocomposites.