Authors: Zirui Qin, Lei Jiao, Lijun Hu, Yiming Zhang, Xiangkun Jia, Chengjie Chen, Xiaolei Sun, Yanling Zhai, Zhijun Zhu, Xiaoquan Lu
Published: 2025-04-25
Source: Full article
AbstractDeveloping efficient peroxidase‐like nanozymes to surpass natural enzymes remains a significant challenge. Herein, an amorphous RuO2 nanozyme with peroxidase‐like activity is synthesized for activating H2O2 with a specific activity of 1492.52 U mg−1, outperforming the crystalline RuO2 nanozymes by a factor of 22 and far superior to natural peroxidases. Amorphous RuO2 nanozymes with long‐range disordered atomic arrangements can effectively elongate the O─O bonds in H2O2. Abundant oxygen vacancies in amorphous RuO2 nanozymes lead to an upshift of the d‐band center, enhancing the exceptional adsorption strength of H2O2, which improve the electron transfer efficiency and ensure superior peroxidase‐like activity. Accordingly, a nanozyme‐linked immunosorbent assay is developed for the precise and sensitive detection of prostate‐specific antigens with a detection limit as low as 0.52 pg mL−1. This study introduces a simple approach for developing high‐performance peroxidase‐like nanozymes to improve the analytical performances of prostate‐specific antigens in clinical diagnostics.