Authors: Isabelle C. Becker, Adrian R. Wilkie, Emma Nikols, Estelle Carminita, Harvey G. Roweth, Julia Tilburg, Anthony R. Sciaudone, Leila J. Noetzli, Farheen Fatima, Genevieve Couldwell, Anjana Ray, Alex Mogilner, Kellie R. Machlus, Joseph E. Italiano
Published: 2024-06-19
Source: Full article
Platelet-producing megakaryocytes (MKs) primarily reside in the bone marrow, where they duplicate their DNA content with each cell cycle resulting in polyploid cells with an intricate demarcation membrane system. While key elements of the cytoskeletal reorganizations during proplatelet formation have been identified, what initiates the release of platelets into vessel sinusoids remains largely elusive. Using a cell cycle indicator, we observed a unique phenomenon, during which amplified centrosomes in MKs underwent clustering following mitosis, closely followed by proplatelet formation, which exclusively occurred in G