Authors: Guojun Shi, Chaoqi Yang, Jiale Wu, Yang Lei, Jiazhi Hu, Jianxun Feng, Qing Li
Published: 2024-08-09
Source: Full article
During lagging strand chromatin replication, multiple Okazaki fragments (OFs) require processing and nucleosome assembly, but the mechanisms linking these processes remain unclear. Here, using transmission electron microscopy and rapid degradation of DNA ligase Cdc9, we observed flap structures accumulated on lagging strands, controlled by both Pol δ’s strand displacement activity and Fen1’s nuclease digestion. The distance between neighboring flap structures exhibits a regular pattern, indicative of matured OF length. While