Authors: Arlin Rodriguez, Bharath Raj Madhanagopal, Kahini Sarkar, Zohreh Nowzari, Johnsi Mathivanan, Hannah Talbot, Akul Patel, Vinod Morya, Ken Halvorsen, Sweta Vangaveti, J. Andrew Berglund, Arun Richard Chandrasekaran
Published: 2025-03-12
Source: Full article
DNA nanostructures are typically assembled by thermal annealing in buffers containing magnesium. We demonstrate the assembly of DNA nanostructures at constant temperatures ranging from 4° to 50°C in solutions containing different counterions. The choice of counterions and the assembly temperature influence the isothermal assembly of several DNA motifs and designed three-dimensional DNA crystals. Molecular dynamics simulations show more fluctuations of the DNA structure in select monovalent ions (Na