Authors: Raluca E. Ghebosu, Lawrence Hui, Joy Wolfram
Published: 2025-01-03
DOI: 10.1186/s12929-024-01095-6
Source: Full article
AbstractThe biomolecular relevance of medium supplements is a key challenge affecting cell culture practice. The biomolecular composition of commonly used supplements differs from that of a physiological environment, affecting the validity of conclusions drawn from in vitro studies. This article discusses the advantages and disadvantages of common supplements, including context-dependent considerations for supplement selection to improve biomolecular relevance, especially in nanomedicine and extracellular vesicle research.